Isolation, Culture, and Transplantation of Muscle Satellite Cells
نویسندگان
چکیده
منابع مشابه
Isolation and optimization of mice skeletal muscle satellite cells using preplating method and culture media substitution
Introduction: Satellite cells are known as the main regenerative cell type in skeletal muscles. Our study established a modified digestion and preplating method for the isolation of slow or weak adherent cells for the enrichment of satellite cells. Low-survival rate of these primary stem cells prompted us to address whether cell culture medium substitution might change cell viability status. M...
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The objective of this study was to establish the optimum protocol for the isolation and culture of porcine muscle satellite cells. Mononuclear muscle satellite cells are a kind of adult stem cell, which is located between the basal lamina and sarcolemma of muscle fibers and is the primary source of myogenic precursor cells in postnatal muscle. Muscle satellite cells are a useful model to invest...
متن کاملisolation and optimization of mice skeletal muscle satellite cells using preplating method and culture media substitution
introduction: satellite cells are known as the main regenerative cell type in skeletal muscles. our study established a modified digestion and preplating method for the isolation of slow or weak adherent cells for the enrichment of satellite cells. low-survival rate of these primary stem cells prompted us to address whether cell culture medium substitution might change cell viability status. me...
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Muscle regeneration in the adult is performed by resident stem cells called satellite cells. Satellite cells are defined by their position between the basal lamina and the sarcolemma of each myofiber. Current knowledge of their behavior heavily relies on the use of the single myofiber isolation protocol. In 1985, Bischoff described a protocol to isolate single live fibers from the Flexor Digito...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2014
ISSN: 1940-087X
DOI: 10.3791/50846